Inhibition studies on tissue cultures of Walker carcinoma 256 and chick embryo heart cells.

Chick embryo heart cells were used in coverslip cultures. The medium consisted of: Chick embryo extract 15% Phenol red solution (.04%) 7% Tyrode solution 28% Chicken plasma 50% Cultures were incubated for 72 hours at 37°C. The amount of inhibition of growth was determined from comparisons of the areas of growth and of the mitotic indexes. Many of the compounds were synthesized in this Laboratory by Doctors Francis B. Cramer, Francis E. Reinhart, and H. A. Rutter. The source is indicated by the initials of the individual followed by (BRF).